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codelink activated microarray slides  (Surmodics IVD)


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    Surmodics IVD codelink activated microarray slides
    Codelink Activated Microarray Slides, supplied by Surmodics IVD, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/codelink activated microarray slides/product/Surmodics IVD
    Average 93 stars, based on 13 article reviews
    codelink activated microarray slides - by Bioz Stars, 2026-03
    93/100 stars

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    Individual heterozygous deletion strains contain two unique barcodes, up-tags (red) and down-tags (blue), flanked by two pairs of common primer sequences (black for all the up-tags, grey for all the down-tags) at the deleted allele. The CaFT pool contains 2,868 strains, representing ∼45% of the C. albicans genome. Aliquots of the pool are treated with an inhibitory compound (at different concentrations) or a mock treatment over 20 population doublings. The relative abundance of each strain is subsequently monitored by <t>DNA</t> <t>microarrays</t> competitively hybridized with amplified and labeled tags (using the common primer pairs) from the two treatments. The response of each strain to the compound is appraised by a normalized z -score, with a positive value indicating hypersensitivity, and a negative value relative resistance.
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    Individual heterozygous deletion strains contain two unique barcodes, up-tags (red) and down-tags (blue), flanked by two pairs of common primer sequences (black for all the up-tags, grey for all the down-tags) at the deleted allele. The CaFT pool contains 2,868 strains, representing ∼45% of the C. albicans genome. Aliquots of the pool are treated with an inhibitory compound (at different concentrations) or a mock treatment over 20 population doublings. The relative abundance of each strain is subsequently monitored by <t>DNA</t> <t>microarrays</t> competitively hybridized with amplified and labeled tags (using the common primer pairs) from the two treatments. The response of each strain to the compound is appraised by a normalized z -score, with a positive value indicating hypersensitivity, and a negative value relative resistance.
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    Average 90 stars, based on 1 article reviews
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    Individual heterozygous deletion strains contain two unique barcodes, up-tags (red) and down-tags (blue), flanked by two pairs of common primer sequences (black for all the up-tags, grey for all the down-tags) at the deleted allele. The CaFT pool contains 2,868 strains, representing ∼45% of the C. albicans genome. Aliquots of the pool are treated with an inhibitory compound (at different concentrations) or a mock treatment over 20 population doublings. The relative abundance of each strain is subsequently monitored by DNA microarrays competitively hybridized with amplified and labeled tags (using the common primer pairs) from the two treatments. The response of each strain to the compound is appraised by a normalized z -score, with a positive value indicating hypersensitivity, and a negative value relative resistance.

    Journal: PLoS Pathogens

    Article Title: Genome-Wide Fitness Test and Mechanism-of-Action Studies of Inhibitory Compounds in Candida albicans

    doi: 10.1371/journal.ppat.0030092

    Figure Lengend Snippet: Individual heterozygous deletion strains contain two unique barcodes, up-tags (red) and down-tags (blue), flanked by two pairs of common primer sequences (black for all the up-tags, grey for all the down-tags) at the deleted allele. The CaFT pool contains 2,868 strains, representing ∼45% of the C. albicans genome. Aliquots of the pool are treated with an inhibitory compound (at different concentrations) or a mock treatment over 20 population doublings. The relative abundance of each strain is subsequently monitored by DNA microarrays competitively hybridized with amplified and labeled tags (using the common primer pairs) from the two treatments. The response of each strain to the compound is appraised by a normalized z -score, with a positive value indicating hypersensitivity, and a negative value relative resistance.

    Article Snippet: We custom-built a set of two DNA microarrays using Amersham CodeLink Activated Slides ( http://www.gelifesciences.com/ ).

    Techniques: Amplification, Labeling